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pro12a  (Cytoskeleton Inc)


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    Cytoskeleton Inc pro12a
    Pro12a, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pro12a/product/Cytoskeleton Inc
    Average 93 stars, based on 1 article reviews
    pro12a - by Bioz Stars, 2026-05
    93/100 stars

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    a. Pro12A GP of live mycoplasma membranes at 37°C and 30°C. The respective GP values of synthetic liposomes are shown for reference. mean +/- SD, n = 3. b . Pro12A GP difference, (37°C-30°C), calculated for synthetic membranes (left), M. mycoides (center) and Syn3B bars show mean difference +/- SD. c . Osmotic senstivity of live mycoplasma cells, grown at 37°C and 30°C.n = 3. The readout shows the absolute slope of salt- propidium iodide fluorescence curves. The curves are plotted on Supplementary Figure Xx.

    Journal: bioRxiv

    Article Title: Chemically defined lipid diets reveal the versatility of lipidome remodeling in genomically minimal cells

    doi: 10.1101/2024.10.04.616688

    Figure Lengend Snippet: a. Pro12A GP of live mycoplasma membranes at 37°C and 30°C. The respective GP values of synthetic liposomes are shown for reference. mean +/- SD, n = 3. b . Pro12A GP difference, (37°C-30°C), calculated for synthetic membranes (left), M. mycoides (center) and Syn3B bars show mean difference +/- SD. c . Osmotic senstivity of live mycoplasma cells, grown at 37°C and 30°C.n = 3. The readout shows the absolute slope of salt- propidium iodide fluorescence curves. The curves are plotted on Supplementary Figure Xx.

    Article Snippet: Pro12A general polarization was calculated according to the formula: Measurements are carried out in Tecan Spark 20 multimode microplate reader, equipped with a thermostat capable of maintaining the temperature with the accuracy of +/- 1°C.

    Techniques: Liposomes, Fluorescence

    a . Membrane lipidome species sorted by abundance from the largest to the lowest, with an average power fit shown as a black line. Dashed vertical lines show the separation point of lipidomes into high- and low- abundance species, using Kneed algorithm (see methods for details). The inset barplots show the average abundance, covered by the high-abundance species in the sample. b . Numberical value for each of the samples average power fit, plotted against the respective lipidome size. c . Average R2 (goodness of fit) plotted against the respective lipidome size for each of the diets. For all scatter plots in a,b and c the markers represent the average value for 6 replicates (3 for 37°C and 3 for 30°C), with respective standard deviations. d . Relative cellular fitness, estimated as the sum of ranks, assigned to each of the experimental readouts in this study (growth rates at 37°C, homeoviscous adaptation as the difference between Pro12A GP values at 37°C and 30°C, osmotic sensitivity at 37°C and 30°C). For details see methods.

    Journal: bioRxiv

    Article Title: Chemically defined lipid diets reveal the versatility of lipidome remodeling in genomically minimal cells

    doi: 10.1101/2024.10.04.616688

    Figure Lengend Snippet: a . Membrane lipidome species sorted by abundance from the largest to the lowest, with an average power fit shown as a black line. Dashed vertical lines show the separation point of lipidomes into high- and low- abundance species, using Kneed algorithm (see methods for details). The inset barplots show the average abundance, covered by the high-abundance species in the sample. b . Numberical value for each of the samples average power fit, plotted against the respective lipidome size. c . Average R2 (goodness of fit) plotted against the respective lipidome size for each of the diets. For all scatter plots in a,b and c the markers represent the average value for 6 replicates (3 for 37°C and 3 for 30°C), with respective standard deviations. d . Relative cellular fitness, estimated as the sum of ranks, assigned to each of the experimental readouts in this study (growth rates at 37°C, homeoviscous adaptation as the difference between Pro12A GP values at 37°C and 30°C, osmotic sensitivity at 37°C and 30°C). For details see methods.

    Article Snippet: Pro12A general polarization was calculated according to the formula: Measurements are carried out in Tecan Spark 20 multimode microplate reader, equipped with a thermostat capable of maintaining the temperature with the accuracy of +/- 1°C.

    Techniques: Membrane